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The purpose of the preface is to explain the books objectives and how to use it give warnings disclaimers and the like The main objective of Protein and Peptide Analysis by Mass Spec trometry is quite straightforwardto present authoritative uptodate and practical accounts of the use of mass spectrometry in the analysis of pep tides and proteins How to use it Every reader will have their own particular interests and will surely be drawn toward the chapters that cover these interests Within the remaining chapters however techniques are described with analytical possibilities that such a reader can then only guess at So read the book fully Again as is customary in the Methods in Molecular Biology series the chapter format Introduction Materials Methods and Notes allows the authors to introduce the techniques to explain their relevance and applicability and above all to provide detaildetail that represents each authors accumulated experience and enables the reader to use and benefit from these methods So read the book fully and read it diligently Warnings and disclaimers Mass spectrometry today offers the pro tein chemist ready access to a wealth of information that is otherwise avail able only with great difficulty or perhaps not at all With this goal in sight any warnings and disclaimers will almost surely be ignored So a warning anyway the use of mass spectrometry might be habit forming
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Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important yet complex analytical technique Unlike many other methods which automatically yield an absolutely unique protein name as output protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products This book enables readers to both understand and appreciate how determinations about protein identity from mass spectrometric data are made Coverage begins with the technical basics including preparations instruments and spectrometric analysis of peptides and proteins before exploring applied use in biological applications bioinformatics database and software resources Citing the most recent and relevant work in the field of biological mass spectrometry the book is written for researchers and scientists new to the field but is also an ideal resource for those hoping to hone their analytical abilities Offers introductory information for scientists and researchers new to the field as well as advanced insight into the critical assessment of computeranalyzed mass spectrometric results and their current limitationsProvides examples of commonlyused MS instruments from Bruker Applied Biosystems JEOL Thermo ScientificThermo Fisher Scientific IU and WatersIncludes biological applications and exploration of analytical tools and databases for bioinformatics
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Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important yet complex analytical technique Unlike many other methods which automatically yield an absolutely unique protein name as output protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products This book enables readers to both understand and appreciate how determinations about protein identity from mass spectrometric data are made Coverage begins with the technical basics including preparations instruments and spectrometric analysis of peptides and proteins before exploring applied use in biological applications bioinformatics database and software resources Citing the most recent and relevant work in the field of biological mass spectrometry the book is written for researchers and scientists new to the field but is also an ideal resource for those hoping to hone their analytical abilities Offers introductory information for scientists and researchers new to the field as well as advanced insight into the critical assessment of computeranalyzed mass spectrometric results and their current limitationsProvides examples of commonlyused MS instruments from Bruker Applied Biosystems JEOL Thermo ScientificThermo Fisher Scientific IU and WatersIncludes biological applications and exploration of analytical tools and databases for bioinformatics
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How to design execute and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry including stepbystep guidelines for sample preparation analysis and data interpretation Michael Kinter and Nicholas Sherman present their own highquality laboratorytested protocols for the analysis of a wide variety of samples demonstrating how to carry out specific experiments and obtain fast reliable results with a 99 success rate Readers will get sufficient experimental detail to apply in their own laboratories learn about the proper selection and operation of instruments and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing Coverage includes Peptide fragmentation and interpretation of product ion spectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquid chromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem mass spectrometry And much more
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